Cmv pcr test sample
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|
| 2 mL | Plasma | EDTA PPT (White) | Centrifuge and refrigerate. | Plasma must be separated from whole blood within 36 hours of collection by centrifugation for samples stored at 2-25 degrees Celsius. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|
| 2 mL | EDTA (Lavender) | Centrifuge, aliquot and refrigerate ASAP. | Plasma must be separated from whole blood within 36 hours of collection by centrifugation for samples stored at 2-25 degrees Celsius. Transfer plasma to a sterile, screw-capped polypropylene tube. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
| 2 mL | Plasma | EDTA (Pink) | Centrifuge, aliquot and refrigerate ASAP. | Plasma must be separated from whole blood within 36 hours of collection by centrifugation for samples stored at 2-25 degrees Celsius. Transfer plasma to a sterile, screw-capped polypropylene tube. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|
| 1 mL | Plasma must be separated from whole blood within 36 hours of collection by centrifugation for samples stored at 2-25 degrees Celsius. Transfer plasma to a sterile, screw-capped polypropylene tube. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Stability
| Environmental Condition | Description |
|---|
| Refrigerated | Store and transport whole blood at 2-25 degrees Celsius. Separate plasma from whole blood within 36 hours. Plasma specimens stored refrigerated (2-8 C) are stable for up to 6 days. |
| Frozen | Plasma specimens may be transported and stored at -20 C. Plasma specimens stored frozen (-20 C) are stable for 12 weeks. Do not freeze specimens in PPT tubes. |
| Ambient | Transport and store whole blood up to 25 degrees Celsius for no longer than 36 hours. Separate plasma from whole blood within 36 hours of collection if stored at temperatures up to 25 degrees Celsius. |
Days Performed
Turnaround Time
Methodology
| Name | Description |
|---|
| Polymerase Chain Reaction (PCR), Quant |
Reference Range
| CMV DNA (copies/mL) |
|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
|---|
| Negative for CMV DNA |
| CMV DNA (IU/mL) |
|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
|---|
| Negative for CMV DNA |
| CMV DNA (log IU/mL) |
|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
|---|
| Negative for CMV DNA |
Special Info
Store and transport whole blood at 2-25 degrees Celsius for no longer than 36 hours. Plasma must be separated from whole blood within 36 hours of collection by centrifugation for samples stored at 2-25 degrees Celsius. Sterile plasma should be transferred to a sterile screw-capped polypropylene tube. Amplification occurs during testing, all handling of specimens must be performed in a sterile manner. Please order in-house CMVQL for qualitative CMV PCR on saliva and urine. Please order send-out CMVCSF for qualitative CMV PCR all other specimen types including CSF, tissue, and bone marrow.
Clinical Info
Cytomegalovirus (CMV) is a common viral pathogen that can cause severe disease in immunocompromised patients. The quantitative cobas CMV is an FDA-approved in vitro quantitative real time PCR (qPCR) assay that targets highly-conserved regions of the CMV DNA polymerase (UL54) gene, and is reported out in international units (IU/mL). It is intended for use as an aid in the management of CMV in solid organ transplant patients and in hematopoietic stem cell transplant patients. In patients receiving anti-CMV therapy, serial DNA measurements can be used to assess viral response to treatment. The results from cobas CMV must be interpreted within the context of all relevant clinical and laboratory findings.
